A Study to Evaluate “Diosgenin” in Laghu gokshur (T.terrestries) and Brihat gokshur (Pedalium murex) by HPTLC Method

Gokshur is one of the plant used in Ayurveda.The plant has diuretic activity.Brihat gokshur and Laghu gokshur are the plants used under the mishrak varga Dashmoola. Both the varieties contains sapogenins,tannins,glycoside etc.. chemical constituents. Among these steroidal saponin is the main active principle which is responsible for the diuretic activity of the plant. The present research work is aimed to find the difference in percentage of Diosgenin in Brihat and Laghu gokshur . A simple, accurate and sensitive HPTLC method was developed for estimation of diosgenin. Toulene : Ethyl acetate : Formic acid (7:2:1) was used as solvent system .The results shows difference in percentage of Diosgenin in two varieties of Gokshur where T. terrestries show 9.2 % and P.murex 8.4 % Of Diosgenin. The correlation coefficient was found to be 0.9989. The developed HPTLC method was simple, accurate, precise and cost-effective & can be utilized for the routine analysis of quantitative determination of Diosgenin.